EPIGENETIC APPROACHES EXPRESS POTENTIAL IN GENERATING EMBRYONIC STEM CELL REPLACEMENTS: A LITERATURE REVIEW
Keywords:
ESC, Epigenetics, TET Dioxygenase, CRISPR-dCas9, Gene Expression, Pluripotent Stem CellsAbstract
Embryonic stem cells (ESCs) have significant potential for curing serious diseases such as Parkinson's and Alzheimer's due to their ability to differentiate into most cell types in the body. However, the extraction of ESCs involves the destruction of embryos, raising moral and ethical concerns. Consequently, many studies have explored the possibility of creating ESC replacements. This literature review examines multiple publications to investigate the potential of epigenetic approaches in inducing stem cells that could replace human embryonic stem cells (hESCs) in biomedical research. Findings indicate that a combination of the demethylation enzyme TET dioxygenase, the CRISPR-dCas9 binding system, and directional guide single-guide RNA (sgRNA) can theoretically revert somatic stem cells to an hESC-like state through DNA demethylation, provided that all key regulators of genetic expression in hESCs are identified.
References
I. Brazier, Yvette. 2018. “What Are Stem Cells, and What Do They Do?” Medicalnewstoday.com. Medical News Today, October 19, 2018. https://www.medicalnewstoday.com/articles/323343.
II. Yang, Wayne et al. “Detection of CRISPR-DCas9 on DNA with Solid-State Nanopores.” Nano Letters 18 (2018): 6469-74 https://pubs.acs.org/doi/10.1021/acs.nanolett.8b02968.
III. Hamilton, James P. “Epigenetics: Principles and Practice.” Digestive Diseases 29 (2011): 130–35. https://doi.org/10.1159/000323874.
IV. Wu, Hao, and Zhang, Yi. “Reversing DNA Methylation: Mechanisms, Genomics, and Biological Functions.” Cell 156 (2014): 45–68. https://doi.org/10.1016/j.cell.2013.12.019.
V. Hematopoietic Stem Cell.” National Cancer Institute. Cancer.gov. 2024. https://www.cancer.gov/publications/dictionaries/cancer-terms/def/hematopoietic-stem-cell.
VI. “Induced Pluripotent Stem Cells | UCLA BSCRC.” Ucla.edu. https://stemcell.ucla.edu/glossary/induced-pluripotent-stem-cells.
VII. “Journal Information | Cell Discovery.” Nature.com. https://www.nature.com/celldisc/journal-information.
VIII. Liang, Gaoyang, and Yi Zhang. “Embryonic Stem Cell and Induced Pluripotent Stem Cell: An Epigenetic Perspective.” Cell Research 23 (2012): 49–69. https://doi.org/10.1038/cr.2012.175.
IX. Lunyak, Victoria V, and Rosenfeld, Michael G. “Epigenetic Regulation of Stem Cell Fate.” Human Molecular Genetics (Print) 17 (2008) (R1): R28–36. https://doi.org/10.1093/hmg/ddn149.
X. Moore, L, Le, T., Fan, G. “DNA Methylation and Its Basic Function.” Neuropsychopharmacol 38, 23–38 (2013). https://doi.org/10.1038/npp.2012.112
XI. Neidhart, Michel. “DNA Methylation – Introduction.” Elsevier EBooks (2016): 1–8. https://doi.org/10.1016/b978-0-12-420194-1.00001-4.
XII. “Oncotarget.” Oncotarget.com. https://www.oncotarget.com/.
XIII. “Oxidative Demethylation.” Rsc.org. 2015. https://www.rsc.org/publishing/journals/prospect/ontology.asp?id=GO:0070989&MSID=b717024f.
XIV. Ralston, Amy, and Shaw, Kenna. “Gene Expression Regulates Cell Differentiation.” Scitable by Nature Education. 2008. https://www.nature.com/scitable/topicpage/gene-expression-regulates-cell-differentiation-931
XV. Salari, Salomeh et al. “Human Embryos Donated for Human Embryonic Stem Cell Derivation.” Fertility and Sterility 119 (2023): 3–10. https://doi.org/10.1016/j.fertnstert.2022.10.023.
XVI. Samrat Roy Choudhury et al. “CRISPR-DCas9 Mediated TET1 Targeting for Selective DNA Demethylation at BRCA1 Promoter.” Oncotarget vol. 7 (2016): 29. https://doi.org/10.18632/oncotarget.10234.
XVII. “Single-Guide RNA (SgRNA).” Innovative Genomics Institute (IGI). Innovative Genomics Institute. September 28, 2020. https://innovativegenomics.org/glossary/single-guide-rna/.
XVIII. SP Medvedev, A.I. Shevchenko, and S.M. Zakian. “Induced Pluripotent Stem Cells: Problems and Advantages When Applying Them in Regenerative Medicine.” Acta Naturae 2 (2010): 18–28. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347549/.
XIX. Xu Xingxing et al. “A CRISPR-Based Approach for Targeted DNA Demethylation.” Cell Discovery 2 (2016). https://doi.org/10.1038/celldisc.2016.9.
XX. Xu, Yuanyuan, and Zhanjun Li. “CRISPR-Cas Systems: Overview, Innovations and Applications in Human Disease Research and Gene Therapy.” Computational and Structural Biotechnology Journal 18 (2020): 2401–15. https://doi.org/10.1016/j.csbj.2020.08.031.
XXI. Zakrzewski, Wojciech et al. “Stem Cells: Past, Present, and Future.” Stem Cell Research & Therapy 10 (2019). https://doi.org/10.1186/s13287-019-1165-5.
XXII. Zhong, Jiang F et al. “Gene Regulation Networks Related to Neural Differentiation of HESC.” Gene Expression 14 (2007): 23–34. https://doi.org/10.3727/000000007783991781.
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